通用转录因子IIF亚基2/TFIIF RAP 30抗体
规格：1mg/1ml
英文名： GTF2F2
别名： ATP dependent helicase GTF2F2; ATP-dependent helicase GTF2F2; BTF4; General transcription factor IIF 30 kDa subunit; General transcription factor IIF polypeptide 2; General transcription factor IIF su
分子量： 28kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human GTF2F2
交叉反应：Human, Mouse, Rat, Cow, Horse, Rabbit,
细胞定位：细胞核
通用转录因子IIF亚基2/TFIIF RAP 30抗体产品介绍：background: Transcription initiation factor IIF subunit beta; In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. TFIIF, a heteromer composed of a small (RAP 30) and a large通用转录因子IIF亚基2/TFIIF RAP 30抗体 (RAP 74) subunit, is required for RNA polymerase II to assemble into a preinitiation complex formed by promotor DNA and the general factors TFIID, IIA and IIB. In addition, TFIIF stimulates transcription elongation by RNA polymerase II. Function: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB.通用转录因子IIF亚基2/TFIIF RAP 30抗体 It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the TFIIF beta subunit family. Gene ID: 2963 Database links: Entrez Gene: 2963 Human Entrez Gene: 609164 Dog Entrez Gene: 68705 Mouse Entrez Gene: 81674 Rat Omim: 189969 Human SwissProt: P13984 Human SwissProt: Q8R0A0 Mouse SwissProt: Q01750 Rat Unigene: 654582 Human Unigene: 20415 Mouse Unigene: 11000 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
通用转录因子IIF亚基2/TFIIF RAP 30抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 转录调节因子 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid