细胞分裂周期样蛋白20抗体
规格：1mg/1ml
英文名： FZR1
别名： CDC20 like 1b; CDC20 like protein 1; CDC20-like protein 1; CDC20C; Cdh 1; CDH1; Cdh1/Hct1 homolog; Fizzy related protein 1; Fizzy related protein 1; Fizzy related protein; Fizzy related protein homolo
分子量： 55kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human FZR1/CDC
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep,
细胞定位：细胞核 细胞浆
细胞分裂周期样蛋白20抗体产品介绍：background: Fizzy-related protein, known as fzr, is a conserved eukaryotic gene that has been recently identified as a 7WD domain family member and is implicated in cell cycle regulation of Drosophila and yeast. Retroviral overexpression of fzr in B-lymphoma cells reduces tumor formation. Fzr overexpression increases B-lymphoma cell susceptibility to natural killer cell (NK) cytotoxicity. Fzr has been implicated in a new category of genes which suppress B-cell tumorigenesis. Current research suggests a novel role for fzr in the target cell interaction with NK cells. Fzr also negatively regulates the levels of cyclins A, B and B3. Loss of fzr causes progression through an extra division cycle in the epidermis and inhibition of endoreduplication in the salivary gland, in addition to failure of cyclin removal. Conversely, premature fzr overexpression downregulates mitotic cyclins, inhibits mitosis and transforms mitotic cycles into endoreduplication cycles. Function: Key regulator of ligase activity 细胞分裂周期样蛋白20抗体of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Subunit: The unphosphorylated form interacts with APC/C during mitosis. Interacts with NINL. Interacts (in complex with the anaphase promoting complex APC) with MAD2L2; inhibits FZR1-mediated APC/C activation. Interacts with USP37. Interacts (via WD repeats) with MAK. Subcellular Location: Cytoplasm and Nucleus. Tissue Specificity: Isoform 2细胞分裂周期样蛋白20抗体 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues. Post-translational modifications: Phosphorylated during mitosis, probably by maturation promoting factor (MPF), leading to its dissociation of the APC/C. Following DNA damage, it is dephosphorylated by CDC14B in G2 phase, leading to its reassociation with the APC/C, and allowing an efficient G2 DNA damage checkpoint. Similarity: Belongs to the WD repeat CDC20/Fizzy family. Contains 7 WD repeats. Database links: Entrez Gene: 51343 Human Entrez Gene: 56371 Mouse Omim: 603619 Human SwissProt: Q9UM11 Human SwissProt: Q9R1K5 Mouse Unigene: 413133 Human Unigene: 24202 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
细胞分裂周期样蛋白20抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 细胞周期蛋白 泛素
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid