Rho相关蛋白激酶1抗体
规格：1mg/1ml
英文名： ROCK1
别名： p160 ROCK1; p160ROCK; Renal carcinoma antigen NY REN 35; Rho associated coiled coil containing protein kinase 1; Rho associated protein kinase 1; Rho-associated coiled-coil containing protein kinase 1
分子量： 158kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human ROCK1
交叉反应：Human, Mouse, Rat, Rabbit,
细胞定位：细胞浆 细胞膜
Rho相关蛋白激酶1抗体产品介绍：background: This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]. Function: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for Rho相关蛋白激酶1抗体centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Subunit: Homodimer. Interacts with RHOB, RHOC, MYLC2B snd PTEN (By similarity). Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM,Rho相关蛋白激酶1抗体 JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner. Subcellular Location: Cytoplasm. Cytoplasm, cytoskeleton, centrosome, centriole (By similarity). Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Note=Associated with the mother centriole and an intercentriolar linker (By similarity). A small proportion is associated with Golgi membranes. Tissue Specificity: Detected in blood platelets. Post-translational modifications: Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR. Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain. Contains 1 REM (Hr1) repeat. Gene ID: 6093 Database links: Entrez Gene: 6093 Human Entrez Gene: 19877 Mouse Entrez Gene: 81762 Rat Omim: 601702 Human SwissProt: Q8MIT6 Cow SwissProt: Q13464 Human SwissProt: P70335 Mouse SwissProt: Q63644 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ROCK1也是丝/苏氨基酸激酶的一种，可调节细胞因子，促进平滑肌收缩和肌动蛋白的形成,因此又称：含有卷曲螺旋的蛋白激酶。目前主要用于肿瘤浸润、转移方面的研究。
Rho相关蛋白激酶1抗体产品应用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 细胞生物 信号转导 细胞周期蛋白 激酶和磷酸酶 细胞骨架
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid